pMEV2HA-AKT1-KAAA

pMEV2HA-AKT1-KAAA encodes a dominant-negative form of AKT1 combining three critical mutations: K179A, T308A, and S473A. This triple mutant lacks catalytic activity and phosphorylation capability, making it a potent inhibitor of endogenous AKT1 signaling in transfected mammalian cells. The vector features an HA-tag for convenient detection and is an essential tool for exploring growth factor resistance, apoptotic regulation, and protein-protein interactions in the PI3K/AKT signaling cascade. This clone is widely used in cancer research, cell biology, and pathway mapping. - 20 ug